Developing an efficient way to freeze and store living tissues could transform many aspects of medical care and research
Developing an efficient way to freeze and store living tissues could transform many aspects of medical care and research, but ice crystallization often occurs within cells during such cryopreservation procedures, leading to cell death. In the November 5 issue of the Biophysical Journal, a Cell Press publication, researchers report that they have gained new information about the processes that are responsible for promoting the freezing of cells within tissues. This knowledge may ultimately lead to novel approaches for preventing tissue injury during cryopreservation.
A long-standing obstacle to avoiding tissue damage during freezing is that when cells are joined together within tissues, individual cells are more likely to crystallize than if the cells are kept apart. “In tissues, ice crystals are thought to be able to grow through membrane channels called gap junctions, thus allowing ice to easily propagate from cell to cell,” explains senior author Dr. Jens Karlsson, of the Department of Mechanical Engineering at Villanova University. “But the results of the present study indicate that the mechanism of tissue cryo-injury is much more complex than was previously thought.”